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KMID : 0379119940220030266
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Korean Journal of Mycology
1994 Volume.22 No. 3 p.266 ~ p.275
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Studies on the Submerged Culture of Lentinula edodes Mycelia in Brewer´s Yeast Extract Medium
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Abstract
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Brewer´s yeast extract can be used as a goad substrate for the culture of Lentinula edodes Mycelia(LEM). We found that it was better to filter the extract thorough three kinds of and than to heat for hydrolysis and concentration at 90℃ prior to use. Also the maximum condition for the growth of LEM was investigated. We found that addition of inorganic salts such as dim enhanced the growth of LEM. On the other hand, addition of carbon and nitrogen souses to the medium did not affect, and even inhibited under certain conditions, the growth of LEM. The maximum temperature for the growth of LEM was around 25℃. Also, it grows better when agitated by shaking at 100 rpm for airration. The appropriate concentration of the extract to use was 10% Under these conditional, LEM could reach bo the confluency after cultivation of 12 days. Our extract formula seems better than other available media far LEM growth, producing higher crude protein content and better taste.
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